I have 375ml of 2206 slurry from 9/24/14. I want to brew a dunkel 1.056 OG on Wednesday.
Since I’ve waited so late to make the starter my only option is to make it about 12 hours before I brew and pitch the whole starter at high krauesen.
With a one step 2L starter I would need 3 new packs of yeast according to mrmalty.
MrMalty says my slurry is only 10% viable. At the highest concentration of yeast it says I need 1100ml of slurry.
Yeastcalc using the middle of the road concentration of yeast and mid range trub says I need 230ml of ‘washed yeast.’ Yeast calc doesn’t account for the age of the ‘washed yeast?’
So I’m thinking of making a 2.5L starter and pitching the 375ml of slurry to it then pitching the starter at high K.
Hmmm. I hope I’m not heading you in the wrong direction, but on Saturday I pitched a 500ml slurry of 1056 that I saved on 9/22 directly into a pale ale at the same OG as yours and it’s going nuts right now. So I’d say you’re probably safe pitching your slurry into a 2.5l starter as you propose.
I put your basic numbers in Brewers Friend calculator. 5.5 gallons 1056 OG 1 billion per ml 62% viability Pro Brewer 1.5 lager target 431 billion cells 1.5 liter starter 436 billion cells
You may have a greater cell density if the slurry is very clean.
[quote=“flars”]I put your basic numbers in Brewers Friend calculator. 5.5 gallons 1056 OG 1 billion per ml 62% viability Pro Brewer 1.5 lager target 431 billion cells 1.5 liter starter 436 billion cells
You may have a greater cell density if the slurry is very clean.
Yes. I get the viability % as if it were a pack of liquid yeast. In the case of your yeast production date of 9/24, the viability was 62%. I multiplied the 375 ml of yeast slurry on hand by 0.62, to approximate the number of viable cells in the 375 ml. I entered .232, 62% of 375, liters of slurry to calculate the starter size.