I’ve been unsuccessfully looking online for the yeast starter chart that shows how many hundred billion yeast cells in a certain volume of starter propagates a certain amount of yeast after 24 hours. I’ve seen the chart, I just can’t find it anywhere. I’m doing a 1.060 American Pilsner and need to do a step starter to propagate the correct amount of yeast to pitch. I just can’t find how much volume I should use for my 2 steps with a 2L flask and 2 smack packs of yeast. I’m guessing it’s around 1600ML with 2 smack packs for the first step, decant and then add another liter or so fresh starter to the flask to get my 450 bil. cells.
FWIW, I ran it through MrMalty and with a stir plate it’s recommending a 2.2 L single-stage starter. You should be fine with just filling the flask as much as you’re comfortable. 3.4 L with “intermittent shaking”, so you could also just use a gallon jug. I wouldn’t want to do two stages when I only needed to slightly more than double the cell count.
[quote]FWIW, I ran it through MrMalty and with a stir plate it’s recommending a 2.2 L single-stage starter. You should be fine with just filling the flask as much as you’re comfortable.[/quote] <–This
You should also be prepared for some blow-off with this scenario (as has been my experience), so a little fermcap might be helpful.